Skip to main content
. Author manuscript; available in PMC: 2020 Mar 2.
Published in final edited form as: Cell Rep. 2020 Feb 18;30(7):2360–2373.e5. doi: 10.1016/j.celrep.2020.01.055

Figure 2. Dorsal CA3 Excitatory Projections to CA1 Instruct Fear Responses to Certain but Not Ambiguous Contextual Threats.

Figure 2.

(A and B) Schematic of the behavioral timeline.

(C) Schematic illustrating dorsal CA3 infection with CaMKII-eNpHR3.0 and fiber optic implantation on top of dorsal CA1 in C57B6/J mice.

(D–G) Silencing dorsal CA3 terminals in dorsal CA1 has no effect on locomotor behavior and innate anxiety in OF (D and E), EPM (F), and NSF (G).

(H and I) Silencing dorsal CA3 terminals in dorsal CA1 decreases freezing behavior in context A but not B (H), which results in a decrease in fear discrimination ratio on block 7 (I). Data (means ± SEM; n = 14, 15 mice per group) were analyzed using mixed factor two-way ANOVA (repeated measure over time) followed by Bonferroni’s multiple comparisons post hoc test and log-rank (Mantel Cox) test. *p < 0.05, eNpHR3.0 versus eYFP; #p < 0.05, block 7 versus block 6. Statistics detailed in Table S1.

(J) Schematic representation of the effect of light silencing dorsal CA3 terminals in CA1 on brain-wide c-Fos expression 60 min following exposure to context A (day 21). Regions highlighted in red denote a significant effect of eNpHR3.0, and arrows indicate the direction of the effect.

See also Figures S1, S3, and S4.