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. Author manuscript; available in PMC: 2020 Mar 2.
Published in final edited form as: Cell Rep. 2020 Feb 18;30(7):2360–2373.e5. doi: 10.1016/j.celrep.2020.01.055

Figure 6. Optogenetic Silencing of DLS PV Neurons Increases Contextual Fear Discrimination.

Figure 6.

(A and B) Schematic of the behavioral timeline.

(C) Schematic illustrating infection of DLS PV neurons with DIO-eNpHR3.0 and fiber optic implantation on top of DLS in PV-Cre mice.

(D–G) Silencing DLS PV neurons has no effect on locomotor behavior and innate anxiety in OF (D and E), EPM (F), and NSF (G).

(H and I) Silencing DLS PV neurons decreases freezing behavior in context B but not A (H), which results in an increase in fear discrimination ratio on block 7 (I). Data (means ± SEM; n = 8,8 mice per group) were analyzed using mixed factor two-way ANOVA (repeated measure over time) followed by Bonferroni’s multiple comparisons post hoc test and log-rank (Mantel Cox) test. *p < 0.05, eNpHR3.0 versus eYFP. Statistics detailed in Table S1.

(J) Schematic representation of the effect of light silencing PV neurons in DLS on brain-wide c-Fos expression 60 min following exposure to context B (day 21). Regions highlighted in red denote a significant effect of eNpHR3.0, and arrows indicate the direction of the effect.

See also Figures S1 and S8.