Table 1.

PDT treatment groups categorized based on the PDT dose and the amounts of [¹O₂]_rx generated during the treatment. All mice were given 5 mg kg⁻¹ Photofrin via tail vein injection (drug-light interval 24 h), and were treated with 630 nm wavelength. This protocol was formulated so that SOED₁ for all mice ≥1.1 mM, but a few mice fell below that requirement after final data analysis of Photofrin concentration after PDT. Interstitial Photofrin concentration, initial tissue oxygenation ([³O₂]₀), in-air light fluence rate (ϕ_air), and treatment time have been shown for each group of mice.

SOED1a	SOED2b	PDT dosec (μMJ cm−2)	Photofrin (μM)	[3O2]0 (μM)	ϕair (mW cm−2)	Timed (s)
0.734 ≤ [1O2]rx ≤ 1.1 mM	0.366 ≤ [1O2]rx ≤ 0.734 mM	400–800	2.2	14.6 ± 3.0	75	6200
			2.5	5.3 ± 3.2		7200
			1.9	17.6 ± 2.0
			2.4	0.8 ± 0.1
			2.8	3.3 ± 0.2	50	7200
			2.3	59.4 ± 16.8
			1.9	41.8 ± 5.0	75
			2.7	2.2 ± 0.3	50
1.1 mM ≤ [1O2]rx			3.0	14.8 ± 4.5	50	7200
			2.9	52.0 ± 13.0
	0.734 ≤ [1O2]rx ≤ 1.1 mM		3.7	42.0 ± 8.0
		1200 ≤	4.9	8.5 ± 2.5	75
			3.7	32.5 ± 7.3
			9.1	14.9 ± 5.0		3000
	1.1 mM ≤ [1O2]rx		5.9	26.1 ± 7.0	50	7200
			5.3	48.8 ± 8.9
			5.8	20.3 ± 5.2
			7.9	20.3 ± 4.1	75	5400
			4.9	2.0 ± 1.0		7200
			5.4	25.2 ± 3.0	50	5400
		800–1200	6.2	13.0 ± 5.0
			4.6	2.6 ± 1.1		7200
			4.3	35.8 ± 6.5
			2.9	26.4 ± 7.0	75
			3.9	10.5 ± 3.0
			3.6	3.5 ± 1.2
			3.7	32.5 ± 7.3
			5.3	48.8 ± 8.9	50

^aSOED₁ groups categorized based on the [¹O₂]_rx values obtained from the measured initial tissue oxygenation [³O₂]₀.

^bSOED₂ groups categorized based on the [¹O₂]_rx values obtained from the whole oxygen spectra measured before and during PDT.

^cPDT dose calculated with the integral of the light fluence rate and Photofrin concentration over time.

^dPDT treatment time.