Figure 5.
Effect of parental exercise on beta cell mass in male offspring at 52 weeks of age. (A) Representative images of insulin-positive areas in sections of pancreas immunostained with anti-insulin antibody (20× magnification). (B) Beta cell mass was calculated by total beta cell area/pancreas area×pancreas weight (mg). (C) Quantification of beta cell size: 10 islets were randomly selected per mouse and 50 cells were measured (μm2) for a total of 500 insulin(+) cells per mouse. (D) Representative immunofluorescence images of pancreas sections stained with anti-glut2 antibody. Images were acquired from all visible multicellular islets at 20× magnification. Data represent mean±SEM. *P<0.05; ***p<0.001 versus Sed. n=6 litters in each group. Mat Ex, maternal exercise; Mat+Pat Ex, both maternal and paternal exercise; Pat Ex, paternal exercise; Sed, sedentary.