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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Biochim Biophys Acta Biomembr. 2020 Jan 15;1862(5):183194. doi: 10.1016/j.bbamem.2020.183194

Figure 4. Characterization of 4F-peptide nanodiscs containing 19F-cytb5.

Figure 4.

Reconstitution of cytb5 in 4F-POPC nanodiscs, 4F-POPC/PS nanodiscs, 4F-POPS nanodiscs and 4F-ER nanodiscs. SEC elution profiles (A, D, G, J) and DLS profiles (B, E, H, K) of cytb5 alone in buffer (red), empty nanodiscs (blue), and nanodiscs containing cytb5 (green). Fits of 19F NMR spectra of 5F-tryptophan-labeled cytb5 reconstituted in 4F-POPC (C), 4F-POPC/PS (F), 4F-POPS (I), and 4F-ER nanodiscs(L) at 310 K.