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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: J Thromb Haemost. 2019 Dec 30;18(3):693–705. doi: 10.1111/jth.14687

Figure 6: Arginines 508, 513, and 530 are critical for the interaction of CDGI with cellular membranes.

Figure 6:

. (A) HA-tagged CDGI (Lane 1; CDGI-HA; ~75 kDa), HA tagged CDGIΔC1 (Lane 2; CDGIΔC1-HA; ~60 kD), or HA-tagged CDGI R508A/R513A/R530A triple mutant (Lane 3; CDGI(R508A/R513A/R530A)-HA) were transfected into GripTite™ 293 MSR cells and assayed for microsome/membrane or cytosolic localization by subcellular fractionation. GAPDH (~40 kDa) and sTransferrin receptor (~110 kDa) were included as a predominantly cytosolic and membrane protein, respectively. (B) Quantification of studies described in (A). Band intensities were determined and expressed as the ratio of membrane fraction to cytosolic fraction (data are mean ± SD; n=3).