Lov-1 cis, noncoding polymorphisms lead to instability of perpetuated silenced state at Lov-1 FLC. (A) Schematic structure of chromosome 5 of Col FRI (gray), Lov-1 (black) and the nearly isogenic line NIL1 (Coustham et al. 2012). Black boxes represent the introgressed Lov-1 genomic segments in NIL1. (B) Expression of FLC mRNA in Col FRI, Lov-1 and NIL1 at 0, 10, 20, and 30 d of growth in the warm after a 4-wk vernalization treatment. Values are means ± SEM of three to six biological replicates. (C) Accumulation of H3K27me3 and H3K36me3 in the same samples and time points as described in B. Values are means ± SEM of two to four biological replicates.