Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 1;34(5-6):413–427. doi: 10.1101/gad.332270.119

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Rahman et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 4. — The NMD-inducing activity of SRSF2 is dependent on the EJC. (A) Western blotting of HeLa cells treated with the indicated siRNAs. Tubulin was used as a loading control. (B) Quantification of knockdown efficiency in A, relative to control siRNA against luciferase (%) (mean ± SD, n = 3). (C) Radioactive RT-PCR of the HBB reporters (WT or T39) of the indicated siRNA-treated HeLa cells cotransfected with the indicated cDNAs. (#) An intron 1 retained transcript variant. (D) mRNA bands in C were quantified, normalized to GFP, and plotted as T39/WT (%) (mean ± SD, n = 3). (*) P < 0.05; (**) P < 0.01; (***) P < 0.001, compared with corresponding empty vector-treated cells, t-test.