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. Author manuscript; available in PMC: 2020 Mar 2.
Published in final edited form as: Acta Biomater. 2019 May 13;95:225–235. doi: 10.1016/j.actbio.2019.05.014

Fig. 3.

Fig. 3.

Printed expansion lattices support proliferation for 1 week. A. Total DNA quantification of cells in single-layer alginate scaffolds indicates a significant increase in cell number over the course of 1 week for bioinks in both low and high concentrations of calcium-supplemented media, 5 mM and 30 mM Ca2+, respectively. NPCs in scaffolds cultured in low calcium-supplemented medium proliferate more than those cultured in high calcium-supplemented medium. All data are normalized to day 0. B. Expansion lattices are printed into a gelatin slurry and contain 4 connected, “S”-shaped layers. Heating is used to release lattices from the slurry, and lattices are cultured in calcium-supplemented medium. C. Total metabolic activity of NPCs in expansion lattices increases after 2 days in culture and remains elevated over 1 week (culture medium included 5 mM Ca2+).