Figure 6.

Effect of SKF 97541 pre‐administration and mid‐administration on sedation time (ST50). Flies were fed with SKF 97451 only for 24 hours prior to the 4‐day tolerance assay (pre‐administration, white bars) or for 24 hours in between the second and third day of the 4‐day tolerance assay (mid‐administration, shaded bars). Horizontal bars represent changes in ST50 in minutes in SKF 97451‐treated flies as compared with control untreated flies for the same day of the tolerance assay. N = 8 (eight vials with eight flies per vial). The ST50 of the control flies is indicated in minutes ± SEM at the zero‐difference point for each consecutive day. Averages ± SEM. 2‐way ANOVA with Tukey vs control of that day. *** = <0.001, **** = <0.0001