Figure 1.
Cryo-TEM images of 10 mM liposomes in D2O composed of (a) POPC, (b) 90 mol % POPC + 10 mol % cholesterol, and (c) 70 mol % POPC + 30 mol % cholesterol extruded through a polycarbonate membrane with a 50 nm pore radius. (d)–(f) are corresponding histograms of liposome radius measured from cryo-TEM compared with normal distribution based on the mean and the standard deviation of the population. Nonspherical liposomes were excluded. The radius of a multilamellar liposome was measured at the outermost vesicle.