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. 2020 Feb 21;11:235. doi: 10.3389/fimmu.2020.00235

Figure 2.

Figure 2

MiR-124 knockdown worsens DSS murine colitis, shifting the Th1/Th2 balance toward Th2 polarization. Mice were tail-vein injected daily with 10 mg/kg LNA-miR-124 or matched scramble control for 3 days. They were then given 3% DSS in drinking water for 8 consecutive days. Body weight change (%) (A), DAI score (B), colon length (C), and HE score (E) were analyzed as mentioned in Figure 1. (D,F) Representative images of colon measurement and HE staining. N = 15–16 per group. (G) Th1/Th2 (IFN-γ/IL-4) ratio in peripheral blood lymphocytes was analyzed by FCM and Student's t-test, as mentioned in Figure 1. (H) Th1-related cytokines IFN-γ and IL-2 and Th2-related cytokines IL-4, IL-6, and IL-13 in colon tissue homogenates were detected by CBA and ELISA and analyzed by Student's t-test. N = 7–9 per group. (I) Representative Western blots (N = 3) of T-bet and GATA3 and corresponding quantification in colon tissues. All data represent mean ± SEM. *p < 0.05, **p < 0.01 vs. scramble group.