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. 2020 Feb 19;11:239. doi: 10.3389/fmicb.2020.00239

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Copyright © 2020 Gonzalez-Escalona and Sharma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Initial cgMLST NJ phylogenetic tree based on gene differences on the 1159 shared genes (allele-based) of the two C. botulinum genomes generated in this study with the other 107 C. botulinum genomes downloaded from GenBank (Supplementary Table S1). In red fonts are the strains sequenced in this study. The assembly generated from the MiSeq data was labeled as strain_ID_MiSeq_flex and the one generated by the combination of nanopore data (long-reads) and MiSeq (short-reads) was labeled as strain_ID_hyb. Both assemblies from each strain were indistinguishable by the cgMLST analysis.