TABLE 2.

Characteristics of the C. botulinum strains used in this study.

Isolate name	Serotype	Accession no.chromosome	Accession no.plasmids	SRAs	Cluster type	Toxin cluster locationa	ST	Source
CDC51232	A, B	CP031097	CP031096, CP031095	SRR7530166, SRR7530167	ha- orfX + BoNTa4; ha + orfX- BoNTB5	plasmid	7	Clinical
83F	A, B	CP031098	CP031100, CP031099	SRR7532471, SRR7532470	ha + orfX- BoNTb5; ha- orfX + BoNTA1	Chr/oppA; Chr/arsC	4	Environmental

^aLocation: Chr/arsC—chromosome in arsC operon, Chr/oppA—chromosome in oppA/brnQ operon. Accessions in bold indicate where the BoNT cluster are located. CFSAN numbers for CDC51232 are CFSAN034200 and CFSAN034202 for 83F. We provided CFSAN numbers to each strain that is sequenced in our laboratories for easy tracking in the system. CFSAN stands for Center for Food Safety and Applied Nutrition.