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. 2020 Feb 14;56(4):932–944. doi: 10.3892/ijo.2020.4987

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Establishment of TAM-resistant breast cancer cells. (A) TAM-resistant cells exhibited a more elongated, spindle-shaped morphology compared with the respective parental cells. Magnification, ×200. (B) Survival of TAM-resistant cells and the respective parental cells was evaluated using a Cell Counting kit-8 assay following treatment with various concentrations of TAM for 24 h. (C) Western blot analysis (D) and densitometry analysis of ERα and HER2 expression in MCF-7, MCF-7R, T47D and T47DR cells. Data are presented as the means ± standard deviation of the mean of 3 repeats. ^*P<0.05, ^**P<0.01, ***P<0.001 vs. respective parental cells. TAM, tamoxifen.