Fig 3. Detection of genomic cassette and protein expression in the retrovirus-infected human dental pulp cells.

(A) PCR detection of CDK4, Cyclin D1, and TERT expression cassettes in the genomic DNA of EGFP, wild type (WT), K4D, and K4DT cells. PCR products from Tuberous sclerosis type 2 (TSC2) were used as an internal control. (B) Western blot analysis of wild type, K4D, and K4DT cells. The results obtained from anti-Cyclin D1, anti-CDK4, and anti-αtubulin antibody staining are shown.