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. 2020 Mar 2;15(3):e0229996. doi: 10.1371/journal.pone.0229996

Fig 8. Osteogenic differentiation properties of wild type and K4DT cells.

Fig 8

Representative Alizarin Red S staining (A) and immunostaining (B) with anti-osteocalcin antibody (red) and DAPI staining (blue) of wild type and K4DT cells. For the Alizarin Red S staining, cells were cultured in osteoinduction medium for 17 days; 25 days for the osteocalcin staining. Middle panels show high magnification of the boxed regions in the left panels. White arrows indicate the positive expression of osteocalcin.