Table 1. Summary of statistics.
| Table Analyzed | Condition | Statistical Test | p value | DF | R2 | Statistic |
|---|---|---|---|---|---|---|
| 1st Branch | WT | Kruskal-Wallis | <0.0001 | H (3) = 18.44 | ||
| WT: iGCL vs oGCL | Dunn's test | <0.0001 | ||||
| WT: iGCL vs Contralateral | Dunn's test | 0.5977 | ||||
| WT: oGCL vs Contralateral | Dunn's test | 0.0073 | ||||
| WT vs ApoE KO | Kruskal-Wallis | <0.0001 | H (6) = 43.62 | |||
| WT vs ApoE KO: iGCL | Dunn's test | 0.5543 | ||||
| WT vs ApoE KO: oGCL | Dunn's test | 0.9172 | ||||
| WT vs ApoE KO: Contralateral | Dunn's test | 0.2871 | ||||
| ApoE3 vs ApoE4 | Kruskal-Wallis | <0.0001 | H (6) = 46.92 | |||
| ApoE3 vs ApoE4: iGCL | Dunn's test | 0.0061 | ||||
| ApoE3 vs ApoE4: oGCL | Dunn's test | 0.2892 | ||||
| ApoE3 vs ApoE4: Contralateral | Dunn's test | 0.0227 | ||||
| Nodes | WT | Kruskal-Wallis | 0.5029 | H (3) = 1.375 | ||
| WT: iGCL vs oGCL | Dunn's test | 0.8527 | ||||
| WT: iGCL vs Contralateral | Dunn's test | >0.9999 | ||||
| WT: oGCL vs Contralateral | Dunn's test | 0.9441 | ||||
| WT vs ApoE KO | Kruskal-Wallis | <0.0001 | H (6) = 32.02 | |||
| WT vs ApoE KO: iGCL | Dunn's test | 0.0003 | ||||
| WT vs ApoE KO: oGCL | Dunn's test | 0.006 | ||||
| WT vs ApoE KO: Contralateral | Dunn's test | 0.0062 | ||||
| ApoE3 vs ApoE4 | Kruskal-Wallis | <0.0001 | H (6) = 46.08 | |||
| ApoE3 vs ApoE4: iGCL | Dunn's test | 0.0001 | ||||
| ApoE3 vs ApoE4: oGCL | Dunn's test | 0.0001 | ||||
| ApoE3 vs ApoE4: Contralateral | Dunn's test | 0.0003 | ||||
| Total Length | WT | Kruskal-Wallis | 0.1369 | H (3) = 0.1369 | ||
| WT: iGCL vs oGCL | Dunn's test | 0.187 | ||||
| WT: iGCL vs Contralateral | Dunn's test | >0.9999 | ||||
| WT: oGCL vs Contralateral | Dunn's test | 0.2969 | ||||
| WT vs ApoE KO | Kruskal-Wallis | <0.0001 | H (6) = 40.64 | |||
| WT vs ApoE KO: iGCL | Dunn's test | 0.0398 | ||||
| WT vs ApoE KO: oGCL | Dunn's test | 0.002 | ||||
| WT vs ApoE KO: Contralateral | Dunn's test | 0.0008 | ||||
| ApoE3 vs ApoE4 | Kruskal-Wallis | <0.0001 | 5 | 0.0862 | F (5, 474) = 8.945 | |
| ApoE3 vs ApoE4: iGCL | Tukey's HSD | <0.0001 | ||||
| ApoE3 vs ApoE4: oGCL | Tukey's HSD | <0.0001 | ||||
| ApoE3 vs ApoE4: Contralateral | Tukey's HSD | 0.4031 | ||||
| Angle | WT | Kruskal-Wallis | 0.0157 | H (3) = 0.0157 | ||
| WT: iGCL vs oGCL | Dunn's test | 0.0134 | ||||
| WT: iGCL vs Contralateral | Dunn's test | 0.2533 | ||||
| WT: oGCL vs Contralateral | Dunn's test | 0.6427 | ||||
| WT vs ApoE KO | Kruskal-Wallis | 0.0005 | H (6) = 22.25 | |||
| WT vs ApoE KO: iGCL | Dunn's test | 0.2197 | ||||
| WT vs ApoE KO: oGCL | Dunn's test | 0.0927 | ||||
| WT vs ApoE KO: Contralateral | Dunn's test | 0.0154 | ||||
| ApoE3 vs ApoE4 | Kruskal-Wallis | <0.0001 | H (6) = 48.8 | |||
| ApoE3 vs ApoE4: iGCL | Dunn's test | 0.0006 | ||||
| ApoE3 vs ApoE4: oGCL | Dunn's test | 0.0907 | ||||
| ApoE3 vs ApoE4: Contralateral | Dunn's test | 0.0004 | ||||
| Spine density | WT vs ApoE KO | One-way ANOVA | <0.0001 | 3 | 0.4861 | F (3, 258) = 81.33 |
| Ipsilateral: WT vs ApoE KO | Tukey's HSD | <0.0001 | ||||
| Contralateral: WT vs ApoE KO | Tukey's HSD | <0.0001 | ||||
| WT Ipsilateral vs Contralateral | Tukey's HSD | 0.5789 | ||||
| ApoE KO Ipsilateral vs Contralateral | Tukey's HSD | 0.6437 | ||||
| ApoE3 vs ApoE4 | Kruskal-Wallis | <0.0001 | H (4) = 196.4 | |||
| Ipsilateral: ApoE3 vs ApoE4 | Dunn's test | <0.0001 | ||||
| Contralateral: ApoE3 vs ApoE4 | Dunn's test | <0.0001 | ||||
| ApoE3 Ipsilateral vs Contralateral | Dunn's test | >0.9999 | ||||
| ApoE4 Ipsilateral vs Contralateral | Dunn's test | >0.9999 | ||||
| Sholl Analysis | WT: iGCL vs oGCL vs Contralateral | Two-way ANOVA | ||||
| Interaction | <0.0001 | 56 | F (56, 4727) = 2.651 | |||
| Row Factor | <0.0001 | 28 | F (28, 4727) = 220.5 | |||
| Column Factor | <0.0001 | 2 | F (2, 4727) = 12.95 | |||
| WT: iGCL vs Contralateral | Fisher's LSD | p < 0.05, 0.05, 0.01 for each 10μm increment between 30 and 50μm from the soma, p < 0.05 at 110μm from the soma, p < 0.05, 0.01, 0.001 for each 10μm increment between 130 and 150μm from the soma | ||||
| WT: oGCL vs Contralateral | Fisher's LSD | p < 0.05, 0.05 at 110 and 120μm from the soma, p < 0.05, 0.05 at 190 and 200μm from the soma | ||||
| WT: iGCL vs oGCL | Fisher's LSD | p < 0.01, 0.01, 0. 01, 0.05 for each 10μm increment between 30μm and 60μm from the soma, p < 0.01, 0.001, 0.001, 0.001, 0.01, 0.001, 0.01, 0.001, 0.01, 0.01 for each 10μm increment between 100 and 190μm from the soma | ||||
| WT vs ApoE KO: iGCL | Two-way ANOVA | |||||
| Interaction | <0.0001 | 28 | F (28, 3944) = 4.607 | |||
| Row Factor | <0.0001 | 28 | F (28, 3944) = 169.7 | |||
| Column Factor | <0.0001 | 1 | F (1, 3944) = 62.34 | |||
| Fisher's LSD | p < 0.0001; p < 0.05, 0.001, 0.01, 0.001, 0.001, 0.001, 0.001, 0.001, 0.001, 0.001 and 0.05 for each 10μm increment between 70μm and 170μm from the soma | |||||
| WT vs ApoE KO: oGCL | Two-way ANOVA | |||||
| Interaction | 0.2707 | 28 | F (28, 3335) = 1.147 | |||
| Row Factor | <0.0001 | 28 | F (28, 3335) = 122.3 | |||
| Column Factor | <0.0001 | 1 | F (1, 3335) = 71.14 | |||
| Fisher's LSD | p < 0.01, 0.01, 0.001, 0.05, 0.01, 0.01, 0.05, 0.05, 0.05, non-significant, 0.05, 0.05, 0.05 for each 10μm increment between 80μm and 200μm from the soma | |||||
| WT vs ApoE KO: Contralateral | Two-way ANOVA | |||||
| Interaction | <0.0001 | 28 | F (28, 4263) = 3.014 | |||
| Row Factor | <0.0001 | 28 | F (28, 4263) = 203.8 | |||
| Column Factor | <0.0001 | 1 | F (1, 4263) = 74.25 | |||
| Fisher's LSD | p < 0.001; p < 0.05, non-significant, 0.05, 0.05, 0.05, 0.01, 0.001, 0.001, 0.001, 0.001, 0.001, 0.001 and 0.01 for each 10μm increment between 40μm and 160μm from the soma | |||||
| ApoE3 vs ApoE4: iGCL | Two-way ANOVA | |||||
| Interaction | <0.0001 | 27 | F (27, 5068) = 6.679 | |||
| Row Factor | <0.0001 | 27 | F (27, 5068) = 208.4 | |||
| Column Factor | <0.0001 | 1 | F (1, 5068) = 143.9 | |||
| Fisher's LSD | p < 0.01, 0.001, 0.001, 0.001, 0.001, 0.001, 0.001, 0.001, 0.001, 0.001, 0.001, 0.001, 0.001 and 0.05 for each 10μm increment between 50μm and 180μm from the soma | |||||
| ApoE3 vs ApoE4: oGCL | Two-way ANOVA | |||||
| Interaction | <0.0001 | 27 | F (27, 3500) = 3.279 | |||
| Row Factor | <0.0001 | 27 | F (27, 3500) = 162.6 | |||
| Column Factor | <0.0001 | 1 | F (1, 3500) = 151.8 | |||
| Fisher's LSD | p < 0.05, 0.001, 0.001, 0.001, 0.001, 0.001, 0.01, 0.01, 0.001, 0.001, 0.001, 0.001, 0.001, 0.01 and 0.001 for each 10μm increment between 50μm and 190μm from the soma | |||||
| ApoE3 vs ApoE4: Contralateral | Two-way ANOVA | |||||
| Interaction | <0.0001 | 27 | F (27, 4704) = 4.133 | |||
| Row Factor | <0.0001 | 27 | F (27, 4704) = 217.8 | |||
| Column Factor | 0.0419 | 1 | F (1, 4704) = 4.143 | |||
| Fisher's LSD | p < 0.01, 0.001, 0.001 and 0 0.001 for each 10μm increment between 40μm and 70μm from the soma, p < 0.05 at 100μm from the soma, p < 0.01, 0.01, 0.01 and 0.01 for each 10μm increment between 180μm and 210μm from the soma | |||||
DF = Degrees of Freedom; iGCL = Inner Granule Cell Layer, oGCL = Outer Granule Cell Layer, in the ipsilateral dentate gyrus; WT = Wild Type. KO = Knockout. The Sholl Analysis represents the average number of intersections in each condition (Column factor) function of the distance to the soma (Row factor).