Figure 2.

Uptake, CFUs and global transcriptome profile of hMDMs infected with mycobacteria. (a) Percent uptake of M. smegmatis, M. bovis BCG and M. tb R179 by hMDMs at 4 h, the percent uptake was calculated by dividing the number of bacteria taken up per milliliter by number of macrophages in each well., (b) CFUs of mycobacteria with multiplicity of infection (MOI) of 1 at 12- and 24-h post infection, and (c) heatmap of differentially expressed transcripts analyzed by AmpliSeq where hMDMs infected with M. smegmatis, M. bovis BCG or M. tb R179 were compared to uninfected hMDMs. The level of expression of each gene in each sample in comparison to the level of expression of uninfected sample is depicted with a color scale with red as maximum and blue as the minimum expression. M. smegmatis having the maximum red indicates stronger host response as compared to other two strains. Dendrogram indicates sample clustering.

Abbreviations: BCG = Bacillus Calmette–Guerin, CFUs = colony-forming units, hMDMs = human monocyte derived macrophages, M. tb = Mycobacterium tuberculosis.