Figure 3.

miR-140 inhibitor promotes CSFV replication.

SUVECs were transfected with miR-140 inhibitors (200 nM) or inhibitor control (IC) and infected with CSFV (MOI = 0.1). (a) Real-time qRT-PCR analysis of CSFV viral RNA (normalized to β-actin expression) at 12, 24, 36, 48 h post-infection. (b) Quantification of CSFV viral genomic RNA in supernatant at 12, 24, 36, 48 h post-infection. The copy number of CSFV viral genomic RNA is calculated by using the standard curve in (Figure 1(a)). Results are expressed as mean ± SD of three independent experiments. p values were calculated using Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001.