Figure 6.

Rab25 promotes CSFV replication.

(a) Real-time qRT-PCR analysis of Rab25 expression (normalized to β-actin expression) after transfection miR-140 mimics (150 nM) and infected with CSFV (MOI = 0.1). (b) Real-time qRT-PCR analysis of Rab25 expression (normalized to β-actin expression) after transfection of Rab25-GFP or control plasmid (GFP) for 24 h in SUVECs. (c) Immunoblot analysis of Rab25 protein levels. The number means the intensity of Rab25 protein level (top) normalized to that of β-actin. The treatment is the same as (a). (d) The SUVECs were transfected with Rab25-GFP or control plasmid and then infected with CSFV (MOI = 0.1). Real-time qRT-PCR analysis of CSFV viral RNA (normalized to β-actin expression) 24 h and 48 h post-infection. (e) The Rab25 gene was silenced by using shRNA approach. Real-time qRT-PCR analysis of Rab25 expression (normalized to β-actin expression) after transfection of shRab25 (shRab25-1, shRab25-2) or control (shN) plasmid. (f) The SUVECs were transfected with shRab25 (shRab25-1, shRab25-2) or control (shN) plasmid and then infected with CSFV (MOI = 0.1). Real-time qRT-PCR analysis of CSFV viral RNA (normalized to β-actin expression) 24 h and 48 h post-infection. Results in (A, C, D, and E) are expressed as mean ± SD of three independent experiments. p values were calculated using Student’s t-test. Western blots were analyzed and quantified using the Image J software. **P < 0.01, ***P < 0.001. NS, not significant.