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. 2020 Feb 27;14:881–893. doi: 10.2147/DDDT.S231008

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© 2020 Mikaelian et al.

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Survival curves of three human cancer cell lines; bladder squamous cell carcinoma cell line (SCaBER, obtained from ATCC), and two ovarian cancer cell lines, OVCAR-3 and IGR-OV1 (obtained from NCI), following a 24 hr incubation with increasing volumes of polarized Rhopalurus princeps venom (Rp). Cell were plated on 24-well plates (5.0 × 10⁴ cells/well) in a volume of 500 ul medium per well and incubated for 24-hr. Then, the cells were treated with the indicated volumes of the polarized Rp for an additional day. Cell viability was determined by the MTT colorimetric assay. These set of experiments were repeated twice.