Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Recombinant DNA reagent | ATM-LacR-Cherry | Soutoglou and Misteli, 2008 | ||
| Recombinant DNA reagent | CDT1-TagRFP | ThermoFisher | P36237 | |
| Recombinant DNA reagent | CMV-Flag | Toiber et al., 2013 | ||
| Recombinant DNA reagent | MRE11-Flag | Wu et al., 2008 | ||
| Recombinant DNA reagent | MRE11-LacR-Cherry | Soutoglou and Misteli, 2008 | ||
| Recombinant DNA reagent | mRFP-SIRT6 | Kaidi et al., 2010 - retracted | ||
| Recombinant DNA reagent | NBS1-Flag | Wu et al., 2008 | ||
| Recombinant DNA reagent | NBS1-LacR-Cherry | |||
| Recombinant DNA reagent | pcDNA3.1(+)Flag-His-ATM-WT | Addgene | 31985 | |
| Recombinant DNA reagent | pcDNA5-FRT/T0-Flag-53BP1 | Addgene | 52507 | |
| Recombinant DNA reagent | pDEST 3x Flag-pcDNA5-FRT/T0-BRCA1 | Addgene | 52504 | |
| Recombinant DNA reagent | pEGFP-C1-FLAG-Ku70 | Addgene | 46957 | |
| Recombinant DNA reagent | pEGFP-C1-FLAG-Ku80 | Addgene | 46958 | |
| Recombinant DNA reagent | pEGFP-C1-FLAG-XRCC4 | Addgene | 46959 | |
| Recombinant DNA reagent | pEGFP- SIRT6 | Kaidi et al., 2010 - retracted | ||
| Recombinant DNA reagent | pET28 hSIRT6-His | Gertman et al., 2018 | ||
| Recombinant DNA reagent | pHPRT-DRGFP | Addgene | 26476 | |
| Recombinant DNA reagent | pMal-C2-hSIRT6-WT | Gertman et al., 2018 | ||
| Recombinant DNA reagent | pMal-C2-hSIRT6-A13W | This paper | ||
| Recombinant DNA reagent | pMal-C2-hSIRT6-D63H | This paper | ||
| Recombinant DNA reagent | pMal-C2-hSIRT6-D63Y | This paper | ||
| Recombinant DNA reagent | pMal-C2-hSIRT6-H133Y | Gertman et al., 2018 | ||
| Recombinant DNA reagent | pMal-C2-hSIRT6-D188A | This paper | ||
| Recombinant DNA reagent | pMal-C2-hSIRT6-D190W | This paper | ||
| Recombinant DNA reagent | pMal-C2-hSIRT6-I217A | This paper | ||
| Recombinant DNA reagent | pQCXIP-Cherry-LacR | This paper | ||
| Recombinant DNA reagent |
pQCXIP-SIRT6 Core-GFP-LacR | This paper | ||
| Recombinant DNA reagent | pQCXIP-GFP-LacR | Addgene | 59418 | |
| Recombinant DNA reagent | pQCXIP-KU80-GFP-LacR | This paper | ||
| Recombinant DNA reagent | pQCXIP-mSIRT6-Cherry-LacR | This paper | ||
| Recombinant DNA reagent | pQCXIP-mSIRT6-GFP-LacR | This paper | ||
| Recombinant DNA reagent | pQCXIP-mSIRT6-H133Y-GFP-LacR | This paper | ||
| Recombinant DNA reagent | pQCXIP-SIRT1-GFP-LacR | This paper | ||
| Recombinant DNA reagent | pQCXIP-SIRT2- GFP-LacR | This paper | ||
| Recombinant DNA reagent | pQCXIP-SIRT7- GFP-LacR | This paper | ||
| Recombinant DNA reagent | SIRT1-Flag | Zhong et al., 2010 | ||
| Recombinant DNA reagent | SIRT2-Flag | Addgene | 13813 | |
| Recombinant DNA reagent | SIRT3-Flag | Addgene | 13814 | |
| Recombinant DNA reagent | SIRT4-Flag | Addgene | 13815 | |
| Recombinant DNA reagent | SIRT5-Flag | Addgene | 13816 | |
| Recombinant DNA reagent | SIRT6 Core | Tennen et al., 2010 | ||
| Recombinant DNA reagent | mSIRT6-WT-Flag | Zhong et al., 2010 | ||
| Recombinant DNA reagent | SIRT7-Flag | Addgene | 13818 | |
| Recombinant DNA reagent | SNF2H-WT-GFP-LacR | Klement et al., 2014 | ||
| Antibody | Alexa Fluor 488 AffiniPure Donkey Anti-Rabbit IgG (H+L) |
Jackson Immunoresearch | 711-545-152 | IF (1:200) |
| Antibody | Alexa Fluor 594 AffiniPure Donkey Anti-Rabbit IgG (H+L) |
Jackson Immunoresearch | 711-585-152 | IF (1:200) |
| Antibody | Alexa Fluor 488 AffiniPure Donkey Anti-Mouse IgG (H+L) |
Jackson Immunoresearch | 715-545-150 | IF (1:200) |
| Antibody | Alexa Fluor 594 AffiniPure Goat Anti-Mouse IgG (H+L) |
Jackson Immunoresearch | 115-585-062 | IF (1:200) |
| Antibody | 53 BP1 |
Snata-Cruz Biotechnology | sc-22760 | IF (1:300) |
| Antibody | BRCA1 | Snata-Cruz Biotechnology | sc-7298 | WB (1:1000) |
| Antibody | Flag | Sigma-Aldrich | F1804 | IF (1:900), WB (1:1000) |
| Antibody | Flag Beads | Sigma-Aldrich | A2220 | IP |
| Antibody | gamma H2A.X (phospho s139) | abcam | ab2893 | IF(1:3000), WB (1:1000) |
| Antibody | Goat Anti-Rabbit IgG H and L (HRP) | abcam | ab6721 | WB (1:10000) |
| Antibody | Histone H3 | abcam | ab1791 | WB (1:5000) |
| Antibody | Histone H3 (acetyl K56) | abcam | ab76307 | WB (1:1000) |
| Antibody | HSC 70 | Snata-Cruz Biotechnology | sc-7298 | WB (1:1000) |
| Antibody | Ku80 | Cell Signaling | #2180 | WB (1:1000) |
| Antibody | MRE11 | abcam | ab214 | WB (1:1000) |
| Antibody | phospho-ATM (Ser1981) | Cell Signaling | #5883 | WB (1:1000) |
| Antibody | phospho-Histone H2A.X (Ser139) | Millipore | 05–636 | IF (1:1500) |
| Antibody | Rabbit Anti-Mouse IgG H and L (HRP) | abcam | ab97046 | WB (1:10000) |
| Antibody | SIRT6 | abcam | ab62739 | WB (1:1000) |
| Antibody | Tubulin | Merck | MAB1637 | WB (1:1000) |
| Peptide, recombinant protein | SIRT1 Human | PROSPEC | PRO-1909 | DNA binding assay |
| Peptide, recombinant protein | SIRT3 Human | PROSPEC | PRO-462 | DNA binding assay |
| Peptide, recombinant protein | SIRT5 Human | PROSPEC | PRO-1774 | DNA binding assay |
| Peptide, recombinant protein | SIRT6 Human | PROSPEC | PRO-282 | DNA binding assay |