Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | HeLa | ATCC Kaláb et al., 2006 |
clone HeLa 61 | Single cell-derived clone |
| Cell line (Homo sapiens) | HEK293T | ATCC | ||
| Cell line (M. musculus) | Primary cortical neurons (embryonic) | This paper | Harvested from timed pregnant C57BL/6J females | |
| Recombinant DNA reagent | pRSET zzRanQ69L | This paper | pKW1234; pK1097 | E. coli expression of Protein A-tagged human RanQ69L |
| Recombinant DNA reagent | pRSET zzRCC1 | This paper | pKW1907; pK1098 | E. coli expression of Protein A-tagged human RCC1 |
| Recombinant DNA reagent | pRSET Rango-2/α1+linkers | This paper | pK44 | E. coli expression of Rango 2 with importin α1 IBB, optimized for FRET |
| Recombinant DNA reagent | pRSET Rango-2/α1 | This paper | pK188 | E. coli expression of Rango 2 with importin α1 IBB |
| Recombinant DNA reagent | pRSET GFP-AviTag | This paper | pK803 | E. coli expression of GFP, with C-terminal Avitag, used in non-biotinylated form here |
| Recombinant DNA reagent | pRSET Importin β- AviTag | This paper | pKW1982; pK1099 | E. coli expression of WT human Importin β, with C-terminal biotin tag |
| Recombinant DNA reagent | BirA ligase | Avidity.com | AVB101 | E. coli expression of untagged BirA biotin ligase for biotinylation of co-expressed AviTag proteins |
| Recombinant DNA reagent | pRSET YFP-M9-CFP | Soderholm et al., 2011 | pKW1006 | E. coli expression of fluorescent M9, TNPO1 cargo |
| Recombinant DNA reagent | pET30a 6His-S-Importin β(1-876) | Chi et al., 1997 | pKW485 | E. coli expression of WT S-tagged human Importin β |
| Recombinant DNA reagent | pGEX GST-GFP-NLS | Levy and Heald, 2010 | pMD49 | E. coli expression of fluorescent importin α cargo with SV40 NLS |
| Recombinant DNA reagent | pGEX-2TK-Nup100(1-610) | Onischenko et al., 2017 | pKW2960, ID 370 | E. coli expression of truncated GST-tagged GLFG domain of yeast Nup100 (human Nup98 homologue |
| Recombinant DNA reagent | pGEX-2TK-Nup100(1-307) | Onischenko et al., 2017 | pKW2959, ID369 | E. coli expression of GST-tagged GLFG domain of yeast Nup100 (human Nup98 homologue) |
| Recombinant DNA reagent | pGEX-2TK-Nup116(348-458) | Onischenko et al., 2017 | pKW2907, ID350 | E. coli expression of GST-tagged GLFG domain of yeast Nup116 (human Nup98 homologue) |
| Recombinant DNA reagent | pGEX-2TK-Nup116(348-458)F > A | Onischenko et al., 2017 | pKW2908, ID351 | E. coli expression of GST-tagged mutant GLFG domain of yeast Nup116 (human Nup98 homologue) |
| Recombinant DNA reagent | pGEX-2TK Nsp1(497-609) | Yamada et al., 2010 | pKW1609; pK1100 | E. coli expression of truncated, GST-tagged FG domain of yeast Nsp1 (human Nup62 homologue) |
| Antibody | Anti-TDP-43 (3H8) (mouse monoclonal) | Abcam | Cat#: ab104223 | WB (1:2000) |
| Antibody | Anti-Matrin 3 (rabbit monoclonal) | Abcam | Cat#: ab151714 | WB (1:5000) |
| Antibody | Anti-importin α (mouse monoclonal) | BD Bioscience | Cat#: 610485 | WB (1:2000) |
| Antibody | Anti-transportin 1 (mouse monoclonal) | BD Bioscience | Cat#: 558660 | WB (1:1000) |
| Antibody | Anti-Ran (mouse monoclonal) | BD Bioscience | BD:610341 | WB (1:500) |
| Antibody | Anti-FUS (rabbit polyclonal) | Bethyl | Cat#: A300-302A | WB (1:1000) |
| Antibody | Goat anti-rat (goat polyclonal, HRP linked) | Cell Signaling Technology | Cat#: 7077S | WB (1:5000) |
| Antibody | Goat anti-rabbit (goat polyclonal, HRP-linked) | Cell Signaling Technology | Cat#: 7074S | WB (1:5000) |
| Antibody | Horse anti-mouse (horse polyclonal, HRP-linked) | Cell Signaling Technology | Cat#: 7076S | WB (1:5000) |
| Antibody | Anti-RCC1 (rabbit polyclonal) | GeneTex | Cat#: GTX104590 | WB (1:2000) |
| Antibody | Anti-Nup62 (rat monoclonal) | Millipore Sigma | Cat#: MABE1043 | WB (1:500) |
| Antibody | Anti-DDX3X (rabbit polyclonal) | Millipore Sigma | Cat#: HPA001648 | WB (1:1000) |
| Antibody | Anti-importin β (mouse monoclonal) | Millipore Sigma | Cat#: I2534 | WB (1:2000) |
| Antibody | Anti-Nup54 (rabbit polyclonal) | Millipore Sigma | Cat#: HPA035929 | WB (1:250) |
| Antibody | Anti-ribosomal protein 6 (RPS6) (mouse monoclonal) | Santa Cruz Biotechnology | Cat#: sc-74459 | WB (1:1000) |
| Antibody | Anti-Nup133 (mouse monoclonal) | Santa Cruz Biotechnology | Cat#: sc-376699 | WB (1:2500) |
| Antibody | Anti-RanGAP1 (C-5) (mouse monoclonal) | Santa Cruz Biotechnology | Cat#: sc-28322 | WB (1:50) |
| Antibody | Anti-Nup98 (2H10) (rat monoclonal) | Santa Cruz Biotechnology | Cat#: sc-101546 | WB (1:2000) |
| Antibody | Anti-hnRNP A1 (4B10) (mouse monoclonal) | Santa Cruz Biotechnology | Cat#: sc-32301 | WB (1:200) |
| Antibody | Anti-hnRNP A2/B1 (EF-67) (mouse monoclonal) | Santa Cruz Biotechnology | Cat#: sc-53531 | WB (1:200) |
| Commercial assay or kit | miRNeasy kit | Qiagen | Cat#:217004 | |
| Chemical compound, drug | Sypro Ruby Protein Gel Stain | Millipore Sigma | Cat#: S4942 | |
| Chemical compound, drug | SYBR Gold Nucleic Acid Stain | ThermoFisher Scientific | Cat#: S11494 | |
| Chemical compound, drug | Importazole | Millipore Sigma | Cat#: SML0341 | |
| Chemical compound, drug | Alexa Fluor 647 NHS ester | ThermoFisher Scientific | Cat#: A37573 | |
| Chemical compound, drug | Alexa Fluor 488 C5 maleimide | ThermoFisher Scientific | Cat#: A10254 | |
| Chemical compound, drug | EZ-Link Sulfo-NHS-LC-biotin No-weigh format | ThermoFisher Scientific | Cat#: A39257 | |
| Chemical compound, drug | Ni-NTA Agarose | Qiagen | Cat#: 30210 | |
| Chemical compound, drug | Glutathione Sepharose 4B | GE Healthcare | Cat#:17-0756-01 | |
| Chemical compound, drug | Dextran, Texas Red, 10,000 MW | ThermoFisher Scientific | Cat#: D1863 | |
| Chemical compound, drug | Dextran, Texas Red, 40,000 MW | ThermoFisher Scientific | Cat#: D1829 | |
| Chemical compound, drug | Dextran, Texas Red, 70,000 MW | ThermoFisher Scientific | Cat#: D1864 | |
| Chemical compound, drug | Digitonin, high purity | Calbiochem | Cat#: 300410 | |
| Other | Ribo Ruler High Range RNA ladder | ThermoFisher Scientific | Cat#: SM1821 | |
| Other | HIS-Select HF Nickel Affinity Gel | Millipore Sigma | Cat#: HD537 | |
| Other | DNase (RNase free) | Qiagen | Cat#: 79254 | |
| Other | RNase A | ThermoFisher Scientific | Cat#: EN0531 | |
| Other | Glutathione-coated polystyrene particles 6.0–8.0 | Spherotech | Cat#: GSHP-60–5 | |
| Other | Neutravidin-coated polystyrene particles 6.0–8.0 | Spherotech | Cat#: NVP-60–5 | |
| Other | GFP Trap Magnetic Agarose | Chromotek | Cat#: Gtma-20 | |
| Other | Wheat germ agglutinin | Millipore Sigma | Cat#: L0636 | |
| Other | BSA, fatty acid-free | Roche | Cat#: 03117057001 |