Figure 3.

Combination of CF33-hNIS-ΔF14.5 (i.t.) with αPD-L1 (i.p.) delays tumor growth and increases survival of mice. (a) Treament scheme showing timing and routes of treatment. Bilateral tumors were generated in abdominal mammary fat pads of C57BL/6 mice by injecting 10⁵ E0771 cells on one side and 2 × 10⁴ E0771 cells on the other side. Only one tumor/mouse (larger tumor) was injected with 10⁷ PFU of virus and the other (smaller) tumor was left un-injected. αPD-L1 Ab (100 µg) was injected intra-peritoneally. Treatments (n = 7 mice/group) were given on each of experimental days 1, 3 and 5. (b) Average tumor volume for virus-injected and un-injected tumors at different time points with SEM has been plotted and compared. p values were calculated using Two-way ANOVA with Dunnett's test. (c) Mice were euthanized when tumor volume exceeded 2500 mm³ and survival of mice among the treatment groups was compared using log-rank Mantel–Cox test.