Figure 8.

Mice treated with CF33-hNIS-ΔF14.5 alone or in combination develop tumor-specific immunity. Mice that underwent complete tumor regression from Figure 4, and remained tumor free for 30 days, were re-challenged with 10⁵ E0771 cells in the abdominal mammary fat pad of opposite side. Age-matched naïve mice were used as control. (a) Tumor volumes were measured weekly and average tumor volumes have been plotted with SEM. Tumors were detected only in the naïve mice. (b) Mice were euthanized when their tumor exceeded 2500 mm³ in volume, and survival was compared using Kaplan–Meier survival plot. (c) CD3+ T cells were isolated from the mice at the end point and re-stimulated with E0771 cells. Cells were stained with antibodies against CD8 and CD107 and analyzed using flowcytometry. Per cent CD8+ T cells positive for CD107 marker have been plotted with SEM and compared among the groups. Stats: One-Way ANOVA.