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. 2020 Jan 17;7(3):e666. doi: 10.1212/NXI.0000000000000666

Figure 1. Human KLHL11-ab demonstrated in a cell-based assay and by immunoprecipitation.

Figure 1

(A) Reactivity (green) of a representative patient's serum with HEK293 cells expressing KLHL11. No immunoreactivity is observed with a serum control (D). (B and E) Reactivity (red) of a commercial KLHL11-ab. (C and F) Merged reactivities of the indicated samples (patient and control samples) with the commercial KLHL11-ab, showing a perfect colocalization with patients' antibodies. Scale bar = 10 μm. (G) Western blot showing that the indicated patients' serum samples with KLHL11-ab (lanes 2–5) immunoprecipitated KLHL11. Lane 1 shows the immunoprecipitation using the commercial antibody, and lanes 6–9 demonstrate the lack of KLHL11 immunoprecipitation with the indicated control samples. Lane 10 is the lack of immunoprecipitation of KLHL11 using untransfected HEK293 cells and serum of a patient with KLHL11-ab.