Table 1 |.

Single-cell RNA sequencing technologies

Technique	Single-cell isolation method	Reverse transcription method	cDNA amplification method	Throughput (number of cells analysed)	UMI	Refs
Tang et al.	Micropipetting	Poly(A)-based	PCR	101	No	10
inDrop	Droplet-based microfluidics system	Poly(A)-based	IVT	104	Yes	20
Drop-seq	Droplet-based microfluidics system	Poly(A)-based	PCR	104	Yes	21
Seq-Well	Microwells	Template-switching	PCR	104	Yes	22
CytoSeq	Microwells	Poly(A)-based	PCR	105	Yes	23
Div-seq	FACS	Template-switching	PCR	103 (nuclei)	No	24
DroNc-seq	Droplet-based microfluidics system	Template-switching	PCR	104 (nuclei)	Yes	25
STRT	Micropipetting	Template-switching	PCR	102	No	159
CEL-seq	Micropipetting	Poly(A)-based	IVT	102	No	170
SUPeR-seq	Micropipetting	Random primer-based	PCR	102	No	171
Quartz-seq	FACS	Poly(A)-based	PCR	102	No	172
MATQ-seq	Micropipetting	MALBAC primer-based	PCR	102	Yes	162
SMART-seq2	Micropipetting	Template-switching	PCR	102	No	173
MARS-seq	FACS	Poly(A)-based	IVT	103	Yes	161
CEL-seq2	Microfluidic devices	Poly(A)-based	IVT	102	Yes	160
Quartz-seq2	FACS	Poly(A)-based	PCR	103	Yes	174
SMART-seq	Micropipetting	Template-switching	PCR	102	No	175
SC3-seq	Micropipetting	Poly(A)-based	PCR	102	Yes	176

CEL-seq, cell expression by linear amplification and sequencing; CytoSeq, gene expression cytometry; Div-seq, RNA sequencing on divided cells; DroNc-seq, massively parallel single-nucleus sequencing with droplet technology; Drop-seq, droplet sequencing; FACS, fluorescence-activated cell sorting; IVT, in vitro transcription; MALBAC, multiple annealing and looping-based amplification cycles; MARS-seq, massively parallel single-cell RNA sequencing; MATQ-seq, multiple annealing and dC-tailing-based quantitative single-cell RNA sequencing; SC3-seq, single-cell mRNA 3′ end sequencing; seq2, second-generation platform; SMART-seq, switch mechanism at the 5′ end of RNA templates sequencing; STRT, single-cell tagged reverse transcription; SUPeR-seq, single-cell universal poly(A)-independent RNA sequencing; UMI, unique molecular identifier.