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. Author manuscript; available in PMC: 2020 Mar 2.

Published in final edited form as: Mol Carcinog. 2018 Aug 10;57(11):1664–1671. doi: 10.1002/mc.22879

Figure 3. — (A) VEGFR2+ pancreatic β-islet SVR (derivative of SVEN) endothelialcells were irraidated with gamma irradiation (2Gy) viz-a-viz stimulation with Th1effector cytokines and the impact of their stimulation was analyzed on the expression of key proteins involved in eNOSsignaling/angiogenesis at 6 h and 24 h post treatment/irradiation. Shown is a representative western blot from 3 independentexperiments. β-actin was used a loading control. (B) Densitometry quantification of representative western blots shown under A were analyzed by Image J and the data were plotted as a mean of protein/actin ratio. Statistical analysis was conducted using a two-tailed paired t-test with 95% confidence intervals (*p<0.05; **p<0.01).(C)SVR cells cultures were irradiated (2 Gy) and eNOS proteins were quantified using a flurometeric-based method attheindicated time intervals. Similarly, (D) SVR cells were cultured in the presence of conditioned media obtained from naïve or irradiated mouse peritoneal macrophages and levels of eNOS proteins were quantified using a flurometeric-based methodattheindicated time intervals

Figure 3. — (A) VEGFR2+ pancreatic β-islet SVR (derivative of SVEN) endothelialcells were irraidated with gamma irradiation (2Gy) viz-a-viz stimulation with Th1effector cytokines and the impact of their stimulation was analyzed on the expression of key proteins involved in eNOSsignaling/angiogenesis at 6 h and 24 h post treatment/irradiation. Shown is a representative western blot from 3 independentexperiments. β-actin was used a loading control. (B) Densitometry quantification of representative western blots shown under A were analyzed by Image J and the data were plotted as a mean of protein/actin ratio. Statistical analysis was conducted using a two-tailed paired t-test with 95% confidence intervals (*p<0.05; **p<0.01).(C)SVR cells cultures were irradiated (2 Gy) and eNOS proteins were quantified using a flurometeric-based method attheindicated time intervals. Similarly, (D) SVR cells were cultured in the presence of conditioned media obtained from naïve or irradiated mouse peritoneal macrophages and levels of eNOS proteins were quantified using a flurometeric-based methodattheindicated time intervals