Fig. 3.

A representative acute myeloid leukemia case harboring an FLT3-tyrosine kinase domain mutation at D835/I836 tested by fragment analysis and next generation sequencing. a FLT3 mutational analysis by fragment analysis test showing a tyrosine kinase domain mutation at D835/I836; x axis represent the PCR product size in base pair (bp) and y axis represent the fluorescence intensity. The wild-type peak at 151 bp (black arrow) successfully cleaved by restriction enzyme EcoRV and the mutant peak at 222 bp (red arrow) resistant to EcoRV cleavage were indicated. b Next generation sequencing read alignments displayed in Alamut® Visual revealed sequence of the corresponding missense mutation at D835/I836, c.2504 A>T; p.D835V. Note that the FLT3 coding reference sequence is in reverse complementary orientation to the next generation sequencing Binary Alignment Map alignment sequence