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. 2020 Feb 25;8:144. doi: 10.3389/fbioe.2020.00144

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Copyright © 2020 Amato, Giannaccini, Dal Monte, Cammalleri, Pini, Raffa, Lulli and Casini.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative immunofluorescence images of cleaved caspase-3 in sections of retinal explants cultured in control conditions (A), in OS (B), in OS with 1 μM OCT (C) or in OS with 1 μM MNP-OCT (D). Retinal layers are visualized with DAPI counterstain. Scale bar, 50 μm. (E) Quantitative analysis of the number of cleaved caspase-3 immunopositive cells per unit length of retinal section. (F) Quantitative analysis of caspase-3 mRNA expression as evaluated with qPCR. Values are indicated as mean ± SEM. ^∗p < 0.05 and ^∗∗p < 0.01 vs. the respective Ctrl; ^§p < 0.05 vs. the respective OS; n = 3 both in (E,F). GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer.