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. 2020 Mar 2;10:3832. doi: 10.1038/s41598-020-60754-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Lateral lipid packing in the SC lipid model containing either Cer (panel A), or 1-deoxy-Cer (panel B), or 3-deoxy-Cer (panel C), or N-Me-Cer (panel D), along with (d)-LIG, Chol, and CholS. First graphs in each panel show the representative infrared spectra of the methylene scissoring vibrations of the unlabeled lipid mixtures at 32 °C. Second and third graphs in each panel show the CH₂ scissoring vibration (mainly from Cer) and CD₂ scissoring vibrations (d-LIG; dashed line), respectively, of the lipid mixtures with d-LIG at 32 °C. The fourth graphs in each panel show the representative infrared spectra of the methylene rocking vibrations of the unlabeled mixtures at 32 °C. A presence of a CH₂/CD₂ doublet in the spectrum is indicative of an orthorhombic packing. Intensity is given in arbitrary units (a. u.).