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. 2020 Mar 2;10:3797. doi: 10.1038/s41598-020-60733-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sequence logos based on the alignment of PB1 domains from the representative land plants (Marchantia, Physco, Amborella and Arabidopsis). Secondary structures (α-helices and β-sheets) represented on the top are based on the ARF5 structure (PDB ID: 4CHK). Numbers represented in the braces next to the name of the gene family, shows the number of sequences present in all these four species together, and also the number of sequences used for that particular alignment logo. Amino acids are coloured according to the group: ‘PAGFLIMV’, ‘KRH’ and ‘DE’ are shown in ‘purple’, ‘blue’ and ‘red’ colours respectively. All other amino acids are shown in ‘black’. Stars at the bottom represent the key residues on positive (blue) and negative (red) faces, corresponding to Lysine and OPCA motif (D-x-D/E-x-D/E core) respectively.