Figure 2.

Expression patterns of the 10 GhWRKY70 genes from cotton under different hormone treatments and after Verticillium dahliae inoculation. (A–C) Expression changes of the 10 GhWRKY70 genes in roots of Zhongzhimian 2 (V. dahliae-resistant) and Xinluzao 7 (V. dahliae-susceptible) after treatment with (A) SA, (B) MeJA, and (C) ETH. Samples collected at 0, 0.5, 1, 3, 6, 9, 12, and 24 h after hormone treatment were used in real-time (RT)-qPCR. (D) Expression changes of the 10 GhWRKY70 genes in roots of Zhongzhimian 2 (V. dahliae-resistant) and Xinluzao 7 (V. dahliae-susceptible) after inoculation with V. dahliae strain Vd991. Samples collected at 0, 1, 3, 6, 12, 24, 48, and 72 h after inoculation were used in reverse transcription-quantitative PCR. Two-leaf stage cotton seedlings were used in the treatment of hormones or V. dahliae. Each experiment was performed using three independent biological replicates.