Table 2.
PV detection in 1-L grab samples with 500-mL processed by two-phase concentration and measured by the WHO algorithm versus BMFS samples by three different detection methods
| Collection/detection method | SL1 (%) | SL2 (%) | SL3 (%) | n |
|---|---|---|---|---|
| Two-phase | ||||
| (1) Virus isolation on L20B and RD cells followed by ITD (CDC) | 21.4 | 64.3 | 33.3 | 42 |
| BMFS | ||||
| (1) Virus isolation on L20B and RD cells followed by ITD (CDC) | 19.0 | 76.2 | 52.4 | 42 |
| (2) Direct real-time RT-PCR (UP) | 0.0 | 14.3 | 31.0 | 42 |
| (3) Integrated cell culture real-time RT-PCR with PLC/PRF/5, L20B, and BGM cell lines (UP) | 31.0 | 81.0 | 66.7 | 42 |
| After amplification on PLC/PRF/5 cells | 16.7 | 61.9 | 47.6 | 42 |
| After amplification on L20B cells | 23.8 | 69.0 | 45.2 | 42 |
| After amplification on BGM cells | 2.4 | 31.0 | 16.7 | 42 |
PV poliovirus, BMFS bag-mediated filtration system, WHO World Health Organization, SL1 Sabin-like PV type 1, SL2 Sabin-like PV type 2, SL3 Sabin-like PV type 3, RD human rhabdomyosarcoma, ITD intratypic differentiation, CDC Centers for Disease Control and Prevention, RT reverse transcription, PCR polymerase chain reaction, UP University of Pretoria, BGM buffalo green monkey