Figure 4.

Biofilm formation capability of the HPS5 and Δcrp strains at different time points. Biofilm formation was determined by measuring the OD₆₃₀ value of dissolved crystal violet at 12, 24, 36, and 48 h post-incubation. Each strain was tested in 6 wells in a 96-well microtiter plate. All the assays above were performed in triplicate. Bars represent the means ± standard deviation of three independent experiments. Statistical analyses were performed using the multiple t-test. *p < 0.05; **p < 0.01.