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. 2020 Feb 25;7:23. doi: 10.3389/fmolb.2020.00023

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Copyright © 2020 Kashfi, Kelsey, Gang, Call and Gang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of specific metabolites from strain HI33, including: (A) total ion chromatogram from ESI + full scan MS analysis of the ethyl acetate extract from liquid cultures of the strain grown for an extended period as described in section “Materials and Methods,” (B,C) selected ion chromatograms for compounds with m/z 1022.709 and m/z 1036.716, respectively, (D) combined full mass spectrum for the compounds eluting between 10.5 and 12.5 min in the UPLC-MS analysis, and (E,F) the MS^E spectra for the peaks at 11.5 and 11.7 min in the UPLC-MS/MS analysis, respectively, including the structures of pumilacidin B and pumilacidin A (candidate compounds in this cluster, inset) collected from PubChem (Kim et al., 2016).