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. 2020 Feb 25;11:87. doi: 10.3389/fendo.2020.00087

Table 3.

Cell viability as determined by Annexin V-FITC/PI staining assay in bovine in vitro granulosa cells.

α-linolenic acid (ALA) cis-9, trans 11 CLA
Treatments control 20 μM 40 μM 80 μM control 15 μM 30 μM 60 μM
Viable cells (%) 76.4 ± 0.5 70.4 ± 4.1 66.9 ± 2.6 73.7 ± 1.7 78.3 ± 1.8a 75.6 ± 3.9ab 71.7 ± 3.7ab 67.5 ± 2.8b
Apoptotic cells (%) 10.5 ± 3.5 10.8 ± 1.4 12.9 ± 5.4 10.6 ± 2.9 10.5 ± 3.0a 9.8 ± 2.5ab 11.9 ± 2.2ab 16.3 ± 3.1b
Dead cells (%) 9.4 ± 2.5 14.6 ± 4.4 14.1 ± 1.8 11.7 ± 1.0 8 ± 1.6a 11.2 ± 2.6ab 12.2 ± 2.2b 12.4 ± 1.5b

Data analyzed by one-way repeated measure ANOVA, multiple comparison vs. control (n = 3; Dunnett's method). Values are means ± SEM. Different lower case letters on the same row indicate significant differences between treatments (P < 0.05).