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. 2020 Feb 25;11:9. doi: 10.3389/fgene.2020.00009

Figure 5.

Figure 5

Relative expression levels of hsa_circ_0007843, miR-518c-5p, and MMP2 in SW480 cells by RT-PCR and western blotting. (A) Relative expression of miR-518c-5p upon downregulation of hsa_circ_0007843 expression; each bar represents the mean of 3 independent experiments. * P < 0.05. (B) Relative expression of MMP2 upon downregulation of hsa_circ_0007843 expression; each bar represents the mean of 3 independent experiments. * P < 0.01. (C) Relative expression of hsa_circ_0007843 upon overexpression of miR-518c-5p; each bar represents the mean of 3 independent experiments. * P < 0.05. (D) Relative expression of MMP2 upon overexpression of miR-518c-5p; each bar represents the mean of 3 independent experiments. * P < 0.01. (E) Relative expression of hsa_circ_0007843 upon downregulation of MMP2 expression; each bar represents the mean of 3 independent experiments. * P < 0.01. (F) Relative expression of miR-518c-5p upon downregulation of MMP2 expression; each bar represents the mean of 3 independent experiments. * P < 0.01. (G) Corresponding expression of MMP2 protein upon downregulation of hsa_circ_0007843 expression. (H) Corresponding expression of MMP2 protein upon overexpression of miR-518c-5p. Control group (no infection), si_hsa_circ_0007843 group (infected with lentiviral vector expressing hsa_circ_0007843 siRNA), hsa_circ_0007843 group (infected with lentiviral vector expressing hsa_circ_0007843), NC group (infected with lentiviral vector expressing negative control sequence), and miR-518c-5p group (infected with lentiviral vector overexpressing miR-518c-5p).