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Tat alters actin cytoskeleton and PECAM expression. Cells were treated as empty media and HIV-1 Tat containing media. In control cells, actin cytoskeleton (Cy3-red) remains intact, and PECAM expression (green) is as expected mainly localized to the plasma membrane. In Tat treated samples, actin cytoskeleton is disrupted, and PECAM expression and distribution changes to be in the cytoplasm and at the plasma membrane.
