Table 2.

Summary of identified metabolites with significantly different concentrations in microdialysis fluids from tumour compared to BAT, one day prior to cisplatin treatment.

	p-valuea	Fold difference tumour/BATb
Metabolites higher ↑ in tumour vs. BAT, pre-treatment
Glycine	0.00032	7.81
beta-Cyano-L-Alanine	0.00014	4.88
Alanine	0.00043	4.49
Proline	0.00012	4.41
Histidine	0.016	4.39
Proline [+CO2]	0.0011	4.31
Uric acid	0.049	4.12
Ornithine	0.00074	3.66
Asparagine	0.00070	3.08
Lysine	0.0025	3.05
2-Aminobutyric acid	0.000031	2.85
Valine	0.00033	2.80
Aminomalonic acid	0.00070	2.76
Tryptophan	0.0130	2.75
Asparagine [-H2O]	0.00063	2.74
2-oxoisocaproic acid	0.0024	2.73
Arginine	0.0022	2.67
Tyrosine	0.00045	2.60
Ornithine-1,5-lactam (3-Amino-2-piperidone)	0.0055	2.44
Leucine	0.00053	2.42
Phenylalanine	0.00090	2.34
Threonine	0.0024	2.31
Glucoheptonic acid	0.037	2.27
Isoleucine	0.0011	2.27
Citrulline (Ornthine)	0.0021	2.22
5,6-Dihydrouracil	0.0049	1.92
3-hydroxybutyric acid	0.0028	1.79
Serine	0.035	1.61
Beta-alanine	0.0050	1.56
2-Hydroxy-3-methylbutyric acid	0.020	1.41
Nonanoic acid	0.0043	1.36
Lactic acid	0.049	1.32
Metabolites lower ↓ in tumour vs. BAT, pre-treatment
5-carbon sugar alcohol mix (XYLITOL/ARABITOL/RIBITOL)	0.000041	0.20
N-acetyl-L-aspartic acid	0.0017	0.21
Aldopentose mix 1 (XYLOSE/LYXOSE/ARABINOSE/RIBOSE)	0.000081	0.26
Erythritol	0.00093	0.37
Guanosine	0.0014	0.39
Hypoxanthine	0.041	0.40
Inositol, myo	0.023	0.41
Inosine	0.0014	0.43
Ascorbic acid	0.0041	0.44
Ketohexose mix 1 (FRUCTOSE/SORBOSE/TAGATOSE/PSICOSE)	0.046	0.47
N-acetyl-mannosamine	0.023	0.54
Glycero-gulo-heptose	0.025	0.68
Octadecenoic acid, 9-E	0.015	0.69

^aTwo-tailed Student’s t-test. All listed p-values in bold meet the FDR < 0.05 criteria by the Benjamini-Hochberg procedure

^bFold difference ratio before treatment was calculated by dividing average metabolite concentration in tumour with average concentration in BAT