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. 2020 Mar 2;10:3788. doi: 10.1038/s41598-020-60689-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunocytochemistry images obtained following PBMEC staining for DAPI (left panel), ZO-1 (middle panel) and merged (right panel) when grown on bovine collagen (50 µg/mL) and fibronectin (7.5 µg/mL) coated coverslips. (A) PBMEC under static media conditions, (B) PBMEC grown under low flow (275 µL/min) and (C) PBMEC under high flow (550 µL/min) for 48 hours using the QV600. Images were taken using a Leica SP5 TCS II MP confocal microscope. White arrow indicates the direction of flow. Yellow arrows indicate formation of tight junctions.