Fig. 7.

Bcl-6 overexpression rescues compromised H3K27me3 modification-induced T_FH cell differentiation. a Heat map of the canonical T_FH lineage-associated genes in WT T_FH cells and EZH2 KO T_FH cells based on data obtained from the microarray analysis. b Quantitative RT-PCR of selected genes in a (normalized to their expression in WT T_FH cells). c Experimental setup. After treatment with either EPZ6438 or vehicle for 3 days, CD45.1⁺SMARTA (SM) cells transduced with an empty retrovirus or retroviral vector overexpressing Bcl6 were transferred into WT CD45.2⁺ recipients that were subsequently infected with LCMV Armstrong. The adoptively transferred SM cells were analyzed on day 5 postinfection. d Flow cytometry analysis of vehicle/EPZ6438-treated SM cells that were transduced with (hCD2⁺) or without (hCD2⁻) a retrovirus expressing Bcl-6. The numbers adjacent to the outlined areas indicate the percentages of SLAM^loCXCR5⁺ T_FH cells, which are summarized in e. NS not significant; *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 (unpaired two-tailed t-test (b); paired two-tailed t-test (e)). The data were obtained from one experiment with two biological replicates pooled from at least ten mice per group (a), are representative of two independent experiments with three technical replicates pooled from at least ten mice per group (b) or are representative of two independent experiments with at least three mice (e) per group (b; error bars indicate the s.d.)