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. 2019 Mar 25;17(3):261–271. doi: 10.1038/s41423-019-0201-9

Fig. 2.

Fig. 2

CD147 participates in anti-citrullinated protein antibody (ACPA)-induced NLRP3 upregulation and activation. a Representative tissue sections from ACPA+ rheumatoid arthritis (RA) patients, ACPA RA patients, and osteoarthritis patients analyzed by immunohistochemical (IHC) methods to determine the expression of NLRP3 and CD147. Images of IHC staining with irrelevant rabbit or mouse immunoglobulin G as the isotype control are also shown. Scale bar = 100 μm. b Statistical results of interleukin (IL)-1β staining. *p < 0.05 and **p < 0.01. c The real-time PCR and western blotting results indicated successful knockdown of CD147 mRNA and protein expression (by approximately 90% of the original expression levels) by lentiviral transduction. d After CD147 knockdown, peripheral blood mononuclear cell-derived macrophages were stimulated with ACPAs or lipopolysaccharide/ATP. Cells infected with empty virus served as the control. IL-1β production in the culture medium was measured by enzyme-linked immunosorbent assay. e Representative results and statistical analysis of caspase1 (p20), IL-1β (p17), pro-caspase1, pro-IL-1β, NLRP3, p-JNK, p-Akt, and IKK protein expression as determined by western blotting after the indicated treatments. Each experiment was performed at least three times. *p < 0.05, **p < 0.01, and ***p < 0.001 (other groups vs. the first group). SN supernatant, LYS cell lysate