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. 2020 Mar 2;10:3799. doi: 10.1038/s41598-020-60834-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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E193K variant does not influence neuritic tree. (A) We expressed in DIV4 cortical neurons GFP together with empty vector or RFP-LRRK2 wild type or RFP-LRRK2 E193K variant. We processed culture at DIV14 for imaging purposes. Scale bar = 50 μm. (B) The graph reports neurite number, expressed as mean ± SE, n = 12 cells. (C) The graph reports total neurite length, expressed as mean ± SE, n = 12 cells.