Fig. 3.

Experimental verification of predicted elevated glycolytic activity and reduced oxygen consumption in murine HCC. a Basal and post-respiratory chain complex inhibition extracellular acidification rates (ECAR) of isolated HCC cells and primary hepatocytes were measured. (n = 10). b Varying media glucose concentrations do not affect the survival of isolated primary hepatocytes (growing period of 72 h; n = 4). c Media glucose concentrations strongly affect the proliferation of isolated HCC cells. (n = 3). d In vivo pSIRM experiments reveal higher ¹³C-incorporation into lactate in tumours after i.p. injection of ¹³C-glucose. e Metabolic flux analyses on isolated cells show a lowered oxygen consumption rate of HCC cells (n = 10). f The number of mitochondria per µm² of cytoplasm was quantified by electron microscopy. *p < 0.05; **p < 0.005; ***p < 0.0001.