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. 2020 Mar 2;10:3850. doi: 10.1038/s41598-020-60615-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histological characterization of CDAHFD mice model. (a) Histological colorations (top: Hematoxylin/Eosin, low: Sirius Red) of Standard Diet- or CDAHFD-fed mice for 6 or 22 weeks. (b) Example of hepatocyte ballooning in a CDAHFD-fed mice (arrowheads). (c–e) Histological scores: steatosis (c), inflammation (d) and fibrosis (e) as a function of time. The points corresponding to 3, 6 and 9 weeks in (c) and 12 and 15 weeks in (e) are not associated with an error bar because for these points, the values of the scores are identical and the standard deviation is therefore 0. (n = 10–14 for each time point, means ± standard deviation).