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. 2020 Mar 2;10:3850. doi: 10.1038/s41598-020-60615-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hypertrophy of HSC is observed in human hepatic fibrosis. (a) Liver fibrosis and HSC hypertrophy scores on 27 human liver biopsies from FIBROTA cohort. (b) Representative images of collocation of retinoid droplets imaged by fluorescence (RF) and collagen by second harmonic generation (CF/SHG) signal on human liver biopsy from FIBROTA cohort exhibiting F3 liver fibrosis. (c) Merge of retinoid fluorescence (RF) and collagen fibers (CF/SHG) imaged by fluorescence and second harmonic generation microscopy on human liver biopsy with fibrosis due to different etiologies: NASH (F2), Viral Hepatitis C (F2) and alcoholism (F4). Scale bar: 50 µm.