Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 2;10:3836. doi: 10.1038/s41598-020-60849-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

AR-V7 does not regulate PARG levels or activity. (a) LNCaP^AR-V7/pHage cells were plated in medium with 10% CSS. Cells were treated with 50 ng/ml Dox for 0, 2, 5, and 7 days. Protein levels of PAR, PARG, PARP-1, AR, AR-V7, and Tubulin were tested by Western blotting. The experiment was repeated 3 times and a representative blot is shown. (b,c) LNCaP^AR-V7/pHage cells were placed in medium with 10% CSS for 24 hours and treated with 50 ng/ml Dox or 10 nM R1881 overnight as indicated. Recruitment of AR-FL and AR-V7 to PSA promoter (b) and PARG (c) were measured by ChIP-qPCR using Igg as control. (*p ≤ 0.05; ***p ≤ 0.001). In panels b-c, each bar represents an average of 3 biological replicates, the experiment was repeated 3 times.