Figure 5.

Inhibition of PARG in androgen-depleted medium causes DNA damage. (a) LNCaP cells were plated in FBS medium. The following day medium was replaced with one supplemented with CSS and treated with vehicle control, 1 µM of PDDX, 100 µM temozolomide, or both, for 48 hours. Representative western blot shown. (b) LAPC4 cells were plated in FBS medium containing 1 nM R1881. The following day medium was replaced to one with CSS and cells were treated as in (a) for 48 hours. Proteins were extracted and levels of PARG, PAR, γ-H2A.X, and Tubulin were compared using western blotting. Experiments shown in this figure were performed 3 times.